节点文献
大鼠肝、肾细胞色素氧化酶亚单位的分离
Isolation of Cytochrome Oxidase Subunits in Rat Liver and Kidney
【摘要】 细胞色素氧化酶是真核生物细胞呼吸链终末氧化酶,是生物体内能量生成的重要步骤。本文利用高分辨率的SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离了大鼠肝、肾细胞色素氧化酶的亚单位组分。
【Abstract】 Cytochrome oxidase is the terminat oxidase of the respiratory chain in eucaryotic cell. In our experiment the oxidase was solubilized using Triton Ⅹ-100 and deoxycholate and was precipitated at 27%~70% ( NH4)2SO-4. The highly purified oxidase from the rat liver and kidney mitochondria was separated into 12-13 different protein subunits by a highly resolving SDS-PAGE.
【关键词】 细胞色素氧化酶;
亚单位;
线粒体;
终末氧化酶;
【Key words】 Cytochrome oxidase; Subunit; Mitochondria; Terminat oxidase;
【Key words】 Cytochrome oxidase; Subunit; Mitochondria; Terminat oxidase;
- 【文献出处】 哈尔滨医科大学学报 ,Journal of Harbin Medical University , 编辑部邮箱 ,1989年06期
- 【下载频次】21