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考马斯亮蓝法测蛋白质含量用于变应原的标准化

Measurement of Protein Content by Coomssie Brilliant Blue in Allergen Standardization

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【作者】 龙如章; 郑艳; 尹蓉;

【Author】 Long Ruzhang, Zhen Yian, Yin Rong Department of Clinical Immunology, Department of Otolaryngology, West China University of Medical Sciences, Chengdu

【机构】 华西医科大学临床免疫教研室; 华西医科大学耳鼻咽喉科;

【摘要】 采用考马斯亮蓝G250检测17种变应原的蛋白质含量,与重量/体积法对照,发现按后者配制的变应原皮试液蛋白质含量悬殊。认为以蛋白质含量作为衡量变应原浸液强度的标准较为合理。此法简便、快速、重复性好,使变应原的标准化程序简化。本文还探讨了皮试液的合宜浓度。

【Abstract】 Coomassie Brilliant Blue method was adopted to detect the protein content of seventeen kinds of allergens. The protein content was regarded as the standard of the potency of allergen extracts. This method can be used in the examination of a heterogeneous group of allergens for its rapidity, simplicity, good reproducibility, stability and the small consumption of samples.

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