【摘要】 本文采用改性甲醇-水为流动相;Shim-Pack CLC-ODS反相柱,用消炎痛为内标,在UV225nm处检测,建立了高效液相色谱法测定血浆中布洛芬浓度的分析方法。该法快速灵敏,重现性好,相关系数为0.9998,不同浓度水平测定结果的日内变异系数小于2%,检出限为0.1μg/ml,回收率为101.22±1.96%。更多还原

【Abstract】 A HPLC method forthe determination of Ibuprofon in plasmawith Indomethacinum as internal standa-rd has been established. This method wascarried out on an ODS column usingmethanol-0. 05MK₂PO₄ （80:20v/v） withorthophosphoric acid adjusted to pH3.2 asa mobile phase. The procedure is follow:transfer 0.5ml of plasma into a 10ml glasscentrifuge tube. add o. 1ml of the internalstandard （50μg/ml） , add 1.4ml of meth-anol shake for 2-3 minutes and centrifugeor 10min at 26000g （15℃）.Inject 8μlaliqueot of supernatant into the Chrama-tograph using the follewing-conditions:Colum. 0.15m×6.0Φ Shim-Pack CLC-ODS（5μm）; Flow rate, 1ml/min; UV detec-tor 225nm; Column temperature, 50℃. This mathod is simple, rapid, sensiti-tive and has a good lineanity. The absoluterecoveries were found to be 101. 22±1.96%for Ibuprofen, Precision studies for withinday at defferent concentrations providedCV values of less than 1.47%. The chro-matographic elution was 6.15 min. andibuprofen quantities as low as 0.1μg/ml.can be assayed.更多还原