【摘要】 活检正常卵巢标本23例和卵巢癌标本16例（粘液性和浆液性囊腺癌各8例）,分离细胞膜作绒毛膜促性腺激素受体结合反应。结果表明:正常卵巢、粘液性及浆液性卵巢癌之平均最大结合率分别为13.30±2.24％、20.15±5.14％及9.06±6.40％;受体量分别为0.66±0.14×10^-10mol/μg膜蛋白、1.97±1.24×10^-10mol/μg膜蛋白及0.48±0.10×10^-10mol/μg膜蛋白,Kd值分别为10.10±5.50×10^-9mol、25.00±0.29×10^-9mol及16.90±0.14×10^-9mol。更多还原

【Abstract】 Human Chorionic Gonadotropin （HCG）is major physiological luteotropic factors forthe human corpus luteum. The observationsstrongly suggest that the human ovarypossesses a gonadotropin receptor in the cellmembrane. We studied the HCG receptor innormal human ovary and ovarian tumors. Twenty-three human ovarian specimensand 16 ovarian tumor specimens were ob-tained from women patients having gyneco-logical surgery Ovaries were homogenizedand sonicated. The homogenates were cen-trifuged at 2000g for 15 min. After sucrosedensity gradient ultracentrifugation （78000g, 4h） ,two fractions were collected from layerof 33% and interface between 33% and 37%.Thirty μg of ovarian ptotein, 8ng ¹²⁵I-HCGand unlabeled HCG in a final volume of0.5 ml of 0.05 mol/L Tris buffer were incu-bated at 30℃ for 2h. The results were shown in the table.更多还原