【摘要】 作者采用SDS-PAGE、2D-PAGE结合银染色法及免疫印迹技术对五株不同致病力钩体外膜蛋白和脂多糖作比较研兜。SDS-PAGE分析发现三株致病钩体外胰蛋白电泳图谱非常相似,与两株非致病性钩体电泳图谱无相关关系。强毒力017株外膜21.5kd区带的蛋白含量明显高于两株弱毒力钩体。2D-PAGE图谱差异更大,主要表现为pI的分布不同。用抗017株的McAb（LB₁）对五株外膜进行免疫印迹分析。该McAb主要识别三株致病性钩体共有的41kd抗原。三株致病性钩体外膜脂多糖电泳图谱与两株非致病钧体外膜脂多糖电泳图谱迥然不同。更多还原

【Abstract】 We applied SDS-PAGE,2D-PAGE andWestern blot to analyse the outer envelopesprotein and LPS of five strains of lepto-spires.The work would lay foundations fortaxonomy, the development of vaccinationregimens and the elucidation of pathogenicmechanisms. The outer envelope proteins of lepto-spires were analyzed by SDS-PAGE andsilver staining. We found that the proteinprofiles of the pathogenic leptospires werebasically identical.A comparison of the pro-tein profiles of the pathogenic L.with thoseexhibited by two nonpathogenic L. indica-ted that there was no obvious relationshipbetween these organisms and any of the L.interrogans strains examined. The quantityof 21. 5kd protein of strain 017 was greaterthan that of strain 601 and 156. Approximately 200, 225, 238 disinctpolypeptides were detected in the strain017, 601 and 156 in 2D-PAGE by silverstaining respectively. The profiles of2D-PAGE showed obvious differences in pI.The pI of strain 017, 60l and 156 were mainly6.68-7.4, 6.55-6.9, 5.85-7.1 respectively. The21.5kd protein of strain 017 was made up ofsix polypeptides. Our immunoblots revealed that McAb（LB₁） reacted with a 41kd antigen, whichwas common to the three virulent lepto-spires tested. SDS-PAGE profiles of silver stainedouter envelope LPS of pathogenic L. differ-ed greatly from those of the nonpathogenicL.There was a distinct differences betweenstrain patoc Ⅰand 3055. Our studies showedthat each of the five strains of leptospirespossessed characteristic outer envelope LPS,which may be used to identify the genus.species and serovars. of a strain of L.更多还原