【摘要】 以c-fos基因为分子探针,对2例正常人脑和11例人脑原发性肿瘤DNA进行Southern吸印杂交分析,同时对上述部分样品的总RNA进行斑点杂交分析。结果表明:1例多形性胶质母细胞瘤发生c-fos基因的扩增和过强表达;1例室管膜瘤在发生c-fos基因扩增的同时,还发生c-fos基因的重排,提示c-fos基因在人脑原发性肿瘤的恶变过程中起着重要作用,其表达水平与肿瘤细胞的分化程度有着密切关系。更多还原

【Abstract】 The c-fos gene was used as a probe todetect the Bam HI-digested brain DNA andtotal RNA isolated from 2 cases of normalhuman brain and 11 cases of human braintumor by Southern blot analysis and RNAdot hybridization technique. The resultshowed an amplification and over-expres-sion of c-fos gene in one case of glioblasto-ma multiforme.These data suggest that thec-fos gene may take an important role inthe carcinogenesis of human primary braintumor, and the level of c-fos expressionmay be correlated with the degree of differ-entiation of brain tumor cells. We also found that there was a rearran-gement of c-fos gene in one case of epen-dymoma. This suggests that, in ependymo-ma, the c-fos gene may be activated in away different from that in the brain tumorsof astroglia origin.更多还原