【摘要】 大鼠肝微粒体依赖NADP的脂质过氧化反应混合物（LPRM）可诱发人羊膜FL系细胞程序外DNA合成（UDS）。当一定浓度的LPRM（0.7～2.4mg微粒体蛋白/ml）加入¹⁴C—TdR预标记的FL细胞培基时,引起羟基尿素（HU）抗性的程序外³H—TdR掺入,与对照组相比差异显著（P<0.05）,并存在剂量—效应关系。由此可见,脂质过氧化反应混合物中含有诱变物,推测其在癌肿发病中可能有直接作用。更多还原

【Abstract】 The reaction mixture of NADPH-dependent lipid peroxidation of rat liver micro-somes induces"unscheduled DNA synthesis（UDS）" in human amnion FL cells. The re-sults obtained were as follows: （1）Lipid peroxidation of microsomes occurred extensi-vely in the incubation with NADPH and Fe²⁺. （2） In the FL cells, UDS level incres-ed markedly when the cells were incubated in the reaction microsomal lipid perox-idation.The induced UDS were dependent on the extent of the lipid peroxidation.According to the results, it is concluded that some DNA-damaging and mutagenic su-bstances are indeed produced in the degradation process of peroxidized polyunsaturat-ed fatty acids in liver microsomal lipids.更多还原