【摘要】 本文介绍焦锑酸盐法在研究细胞内Ca²⁺的应用。采用Wistar鼠离体心脏钙矛盾模型、Wistar鼠脑缺血与再灌流模型和内毒素诱发兔脑水肿模型,在电镜下对细胞内Ca²⁺进行定位显示,并运用x线显微分析对Ca²⁺进行了验证。此外,本文还对该方法的优缺点,方法的改进和Ca²⁺的鉴定进行了探讨,旨在为揭示Ca²⁺与细胞中毒的发病机制提供新的实验手段。更多还原

【Abstract】 In order to provide a new experimental approach concerning the mechanism of cellular toxicosis with Ca2+, antimonate method was used in three models (Ca2+paradox model of isolated rat heart, ischemia and reperfusion model in Wistar rat and brain edema model induced by typhoid endotoxin in rabbit) to localize intracellular Ca2+and to identify Ca2+deposit combinated with antimonate with x-ray microanalysis at ultrastructural level. The method is easy and effective. An evaluation of the method and methodologic modification is discussed.更多还原