【摘要】 用体外琼脂培养法研究胆碱能受体激动剂和拮抗剂对粒、单造血祖细胞（CFU-GM）增殖和分化的影响。结果显示,10^-10-10^-6氨甲酰胆碱均能增加CFU-GM的产率,阿托品可以阻断这种效应;氨甲酰胆碱亦能增加CFU*GM的自杀率及培养基中粒细胞集落的比例。文中讨论了CFU-GM上胆碱能受体的功能特征。更多还原

【Abstract】 This study observed the effect of cholinergic agonist and antagonist on proliferation and differentiation of rganulocytemacrophage haemopoietic progenitor cells (CFU-GM) in vitro. The results showed dosage dependent enhancement of granulocytemacrophage colony growth after treatment of murine bone marrow cultrue with cholinergic agonist carbamylcholine (Cach) .Exposure of CFU-GM to Cach caused a marked increase in their sensitivity to the cytocidal action of cytosine arabinoside.On the other hand, the proportion of granulocytic colonies , which was identified by staining in situ, among the total CFU-GM colonies was also increased after administration Cach. Atropine but not d-Tubocurarine might abolish the above cell effects of Cach.These data indicate the presence of muscarine receptors on CFU-GM. Through activating muscarinic, receptors, Cach may trigger CFU-GM form Go into DNA synthesis phase, and induce differetiation of CFU-GM towards granulocytic cell line.更多还原