【摘要】 用不同链长的马来酰亚胺氮氧自由基Ⅰ,Ⅱ,Ⅲ,Ⅳ和Ⅴ标记健康人血红细胞膜的ESR波谱表明,在单独作用时,重铬酸钾(Cr⁶⁺),氟化钠（F-）和丝裂霉素C（Mit C）降低W/S值（弱固定化组分/强固定化组分）,而秋水仙素（Colch）则增大;在联合作用时,除保留Cr⁶⁺和Mit C与Colch的不同以外,F-与其它三种诱变剂产生协同效应,尤在链长Ⅰ和Ⅴ较为明显.在以上四种诱变剂作用前后,以下三项均无特异的变化:五种链长自旋标记物标记测得的2A//均值±标准差为66.6±2.85G;W/S值随自旋标记物分子链长的增加而增大,与膜蛋白巯基结合位置呈圆锥形,其深度为15.33A;Ⅰ和Ⅲ链长分子与膜蛋白巯基结合位置的ESR波谱中出现Q峰,Ⅱ的大于Ⅲ的.更多还原

【Abstract】 Healthy human erythrocyte membrane was labeled with various chain len-ghths of maleimide nitroxide I ,Ⅱ,Ⅲ, Ⅵ and V, and their ESR spectra showed. Under the singular action,potassium bichromate (Cr6+), sodiam fluoride (F-) or mitomycin C (Mit C) decreased W/S(weak immobilized component/strong immobilized component) Values,but colchicine(Colch) increased it. Under the combined action,except for the remaining difference between Colch and Cr6+ or Mil C,the effects F- and three other mntagens were cooperative, particularly significant in I and Ⅱ. Before and after the action of above four mutagens, there were no specific change in following three respects. Average 2A//± standard deviation which were labeled with 5 chain lengths of spin label were 66.6±2.85 G; W/S increased with the increase of the chain length, the shape of binding sites between 5 chain lengths and the membrane protein SH were conical, and their depths were more than 15.33A. A Q peak oecurred in ESR spectra in the binding sites between Ⅱ or Ⅲ and SH, in which the Q of Ⅱ was higher than that of Ⅲ.更多还原