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桑树品种过氧化物酶同工酶初步研究
A Elementary Study on Peroxidase Isozymes of Mulberry Varieties
【摘要】 应用聚丙烯酰胺垂直平板凝胶电泳法对属于3个种40个桑树品种的幼叶与成叶共382个样品的过氧化物酶同工酶分夏、秋两期进行检测.结果得出:1.桑树品种过氧化物酶同工酶可以划分为两个酶区.A区相对泳动率(R f)范围为0.06—0.41;B区0.51—0.64.2.夏(六月份),幼叶与各期成叶的过氧化物酶同工酶表现稳定,幼叶比成叶酶带表现丰富.3.A1、A2、A3、A4和A7带与生长发育相关.4.40个桑树品种的主酶带表现相似,根据A9带的表现与否把桑树品种分为两大类群.5.两大类群中均包括鲁桑、白桑、山桑三个种,反映了三个种内的基本一致性.6.根据夏幼叶表现稳定的酶带,在两大类群的基础上,把桑树品种分为五类.
【Abstract】 The peroxidase isozymes in young and mature leaves ( 382 samples) from 40 mulberry varieties belonging to 3 species were tested with polyacrylamide gel electrophoresis on vertical plate in summer and autumn respectively. The conclusions are as following: 1 . The peroxidase isozymes spectrum of mulberry varieties can be divided into 2 zyme regions- region A and region B. The range of relative electrophoretic mobility (Rf) of region A is 0.06-0.41, and that of region B-0.51-0.64. 2 . In summer (June) the manifestation of the peroxidase isozymes in young and mature leaves in every periods is stable. The manifestation of the young leaves is more abundant than that of the mature leaves. 3 . A1, A2, A3, A4 and A7 bands are related to growth. 4 . The manifestation of the main zyme bands in 40 mulberry varieties is similar, and the multerry varieties can be divided into two groups according to whether A9 band is manifested or not. 5 . The both groups include the mulberry species-Morus multicaulis P. , Morus bombycis K. , Morus alba L., which shows the identity in their inner gene. 6 . Mulberry varieties are divided into 5 kinds on the base of the two groups according to the zyme band the manifestation of which in the young leaves is stable in summer.
- 【文献出处】 华北农学报 ,Acta Agriculturae Boreali—Sinica , 编辑部邮箱 ,1989年S1期
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