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甘兰夜蛾核型多角体82—17病毒株对裸鼠感染性的研究

Studies on the Infection of NPV82-17 Strain of Nuclear Polyhedrosis Virus from Mamastra brassicae (Lepidoptera) to Nude Mice

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【作者】 宫芸秀扈利华邢祖培李美茹杨晓峰李红

【Author】 Gong Yunxiu Hu Lihua Xing Zupei(Institute of Plant Protection, Beijing Municipal Academy of Agricultrual and Forestry Sciences, Beijing)Li Meiru Yang Xiaofeng Li Hong(Department of Instrumentation, Beijing Municipal Centre for Hygiene and Epidemic Control, Beijing)

【机构】 北京市农林科学院植环所北京市卫生防疫站北京市卫生防疫站 北京北京北京

【摘要】 甘兰夜蛾核型多角体NPV82—17病毒株,经腹腔、静脉、口服感染裸鼠.被感染裸鼠9—37天的脏器经电镜观察,其肝、脾的枯否细胞和网状细胞的胞浆内都存有1至数个多角体.另将其脏器悬液回接靶昆虫棉铃虫,腹腔和静脉感染率为83—100%,口服感染率为7—77%.

【Abstract】 Nuclear polyhedrosis virus strain, NPV 82-17, from Mamastra brass-icae (Lepidop.) was inoculated intraperitonealy, intravenously and orally into the bodies of nude mice. After 9-37 days, the viscera of the infected mice was examined under electron microscope. One or more polyhedron viruses were discovered in the liver and spleen Kupffer cells, and in the cytoplasm of the reticular cells of the nude mice. Moreover, the suspensions of the infected mice viscera were fed back to the target insects, Heliothis armigera (lepedoptera) larvae. The results showed that the larvae infected with NPV82-17 consisted of 83-100% in the intraperito-neal and intraveaous groups, and 7-77% in the oral group.

【关键词】 裸鼠甘兰夜蛾核型多角体
【Key words】 Nude miceMamastra brassicae NPV
  • 【文献出处】 华北农学报 ,Acta Agriculturae Boreali—Sinica , 编辑部邮箱 ,1989年03期
  • 【被引频次】2
  • 【下载频次】4
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