【摘要】 <正> 关于生物组织传感器的研究,Arnold有详细综述。刀豆中包含丰富的脲酶,本文报道将刀豆切片与自制石墨内导PVC膜pH电极型氨气敏电极偶合制成了刀豆切片脲电极,对其响应特性、介质条件、固定化及切片厚度等因素进行了研究,取得了较满意的结果。更多还原

【Abstract】 A jack bean tissue section electrode sensitive to urea has been prepared by coupling a jack bean slice with an ammonia gas-sensing probe using PVC membrane pH electrode as the internal element. The potentiometric response characteristics of this new electrode under steady-state and dynamic condition were examined. The steady-state linear response ranse of this electrode is 1.0 × 10-2-4.6 ×10-5mol/L, with a detection limit of 4.0 × 10-5mol/L. The influence of some experimental parameters including buffer composition, operation pH, etc. are discussed. Urea was determined by using dynamic method, with an average recovery of 98.9% and a standard deviation of 1.8%.更多还原