节点文献
植物酶在解剖组织中有机磷、氨基甲酸酯农药检测中的应用
APPLICATION OF PLANT HYDRYOLASES IN THE DETERMINATION OF ORGANO-PHOSPHORUS AND CARBAMATE PESTICIDES IN POST-MORTEN SAMPLES
【摘要】 本文报导了有机磷和氨基甲酸酯检测的一个新的酶抑制法.该法基于有机磷和氨基甲酸酯化合物对植物酶水解底物2,6-二氯乙酰靛酚的抑制作用.本法简单,快速,适于农药中毒的现场检验.其最小检出量分别为0.2~1μg/μ1和1~2μg/ml.
【Abstract】 A new enzyme inhibition method for determination of organophosphorus and carbamate acompounds in the post-mortem samples has been developed. The principle of this method is based on the inhibiting action of organophosphorus and carbamate compounds to plant hydrolases which catalyticaily hydrolyze the substrate 2, 6-dich-lorophenolindophenol acetates. The method is simple, rapid and suitable for ensic examination of post-mortem samples for the pesticides poisoning. The minimum detecting amount of organophosphorus and carbamate compounds is 0.2~Img/ml and 1~2mg/ml respectivily.
【关键词】 植物酶;
有机磷农药;
氨基甲酸酯农药;
试管法;
薄层层析法;
【Key words】 Plant hydrolases; Organophosphorus pesticides; Carbamate pesticides Test tube method; TLC;
【Key words】 Plant hydrolases; Organophosphorus pesticides; Carbamate pesticides Test tube method; TLC;
- 【文献出处】 中国法医学杂志 ,Chinese Journal of Forensic Medicine , 编辑部邮箱 ,1989年02期
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