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植物酶在解剖组织中有机磷、氨基甲酸酯农药检测中的应用

APPLICATION OF PLANT HYDRYOLASES IN THE DETERMINATION OF ORGANO-PHOSPHORUS AND CARBAMATE PESTICIDES IN POST-MORTEN SAMPLES

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【作者】 刘久华; 王清福; 施昆;

【Author】 Liu Jiuhua; Wang Qingfu; ShiKun Bureau of public security of Hebei Province, Shijiazhuang

【机构】 河北省公安厅; 河北省公安厅;

【摘要】 本文报导了有机磷和氨基甲酸酯检测的一个新的酶抑制法.该法基于有机磷和氨基甲酸酯化合物对植物酶水解底物2,6-二氯乙酰靛酚的抑制作用.本法简单,快速,适于农药中毒的现场检验.其最小检出量分别为0.2~1μg/μ1和1~2μg/ml.

【Abstract】 A new enzyme inhibition method for determination of organophosphorus and carbamate acompounds in the post-mortem samples has been developed. The principle of this method is based on the inhibiting action of organophosphorus and carbamate compounds to plant hydrolases which catalyticaily hydrolyze the substrate 2, 6-dich-lorophenolindophenol acetates. The method is simple, rapid and suitable for ensic examination of post-mortem samples for the pesticides poisoning. The minimum detecting amount of organophosphorus and carbamate compounds is 0.2~Img/ml and 1~2mg/ml respectivily.

  • 【文献出处】 中国法医学杂志 ,Chinese Journal of Forensic Medicine , 编辑部邮箱 ,1989年02期
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