【摘要】 用狂犬病疫苗为抗原,免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤细胞株SP2/O融合,经过培养、克隆、ELISA鉴定,筛出3株分泌抗狂犬病疫苗单克隆抗体细胞株。双扩散结果证明:3株细胞均分泌IgG1。更多还原

【Abstract】 The spleen cells from BALB/c mice immunized with rabies vaccine （RV） were fused with SP2/O myeloma cells. Three hybridoma cells line (RV-F₈, RV-D₃, RV-D₁₁, ) McAb against RV was obtained after cloning for over two time, its specificity was ide ntified by ELISA. The McAb was identilied as mouse IgG₁.更多还原