【摘要】 对草鱼细胞进行同步化处理,并在DNA复制的早期和晚期分别掺入BrdU,制备的染色体标本置于CaCl₂溶液中温浴同时用紫外线照射,然后用Giemsa染色即可分别显示出G带和R带。标本中的部分晚前期和早中期分裂相具有高分辨染色体显带特征。用这一技术对草鱼每条染色体进行识别和分析,提出了初步的草鱼核型,发现了四对草鱼染色体具有随体。更多还原

【Abstract】 Combining cell synchronization in vitro and bromodeoxyuridine (BrdU) incorporation in early synthesis phases and late synthesis phase of ceils of Cicnopharyngodon idellus, two kinds of chromosome patterns of replication banding, R- and G-bands were gained after chromosome preparation were irradiakd in warm CaCl2 solution by UV and were stained with Gicmsa. Some spreads, lengthened in late-prophasc and prometaphasc show more numbers of bands and charaderisties of higher resolution. Analysis of G-bandcd karyotype shows thai there are four pairs of chromosomes with satellite.A tentative idiogrammatic representation of G-banded karyotype of Cteno-pharyngodon idclhsis proposed, the significance, techniques and possible mccha-nism of chromosome banding of fish arc discussed.更多还原