【摘要】 近两年我们采用一个经过改造,致瘤能力增强的农杆菌新菌系A281,对原来连续几年实验的易结瘤和不易结瘤的大豆基因型进行了致瘤能力和效果的实验。用A281,C58,T37三个菌系对易结瘤的20份大豆基因型进行了致瘤能力的比较;用A281菌系对不易结瘤的88份大豆基因型进行了致瘤效果的观察,使67份不易结瘤的基因型也可结瘤。而对照组只有3份基因型可结瘤。实验证明:农杆菌新菌系A281对易结瘤大豆基因型不但和C58,T37具有相同的致瘤率,并且比其具有更强的致瘤能力。对不易结瘤的大豆基因型,产生了致瘤效果。这使一些不能结瘤的优良大豆类型,通过Ti质粒进行遗传改良成为可能。更多还原

【Abstract】 In last two years, we tested the tumor-inducing ability and effect of a improved, new strain of Agrobacterium tumefaciens with high tumor-inducing ability, A281, on tumor-f orming-easily soybean genotypes and tumor-forming-uneasily ones tested for years.Tumor-inducing abilities of three strains, A281 C58, and T37 were compared on 20 tumor-forming-easily genotypes. Tumor-inducing test of A281 was undertaken on 88 tumor-forming-uneasily genotypes, and 67 tumor-formation genotypes were obtained. However,there were only 3 tumor-formation genotypes in the check group. The results showed that the new strain A281, not only has the same tumor-inducing frequency as C58 and T37, but also has more higher tumor-inducing ability on tumor-forming-ea-sily genotypes. Tumor-inducing effet on tumor-forming-unesily genotypes means that it is possable to improve some tumor-forming-uneasily but good soybean genotypes by using Ti-plasmid.更多还原