【摘要】 对采自安徽、河南两省淮北地区的黄犊牛腹泻粪样进行电镜观察,轮状病毒检出率48.33%;用RIHA检测粪样,阳性率37.1%;PAGE法检查,出现典型轮状病毒核酸电泳图谱,检出率62.5%;用RIHI检测母牛血清中轮状病毒抗体,阳性率86.7%。用牛轮状病毒细胞适应毒进行人工复制疾病试验,获得成功。据此,该病鉴定为轮状病毒病。用牛丙种球蛋白和牛血干扰素试验性治疗,治愈率分别为95.27%和91.89%,疗程均在2天以内。本文还比较了该病病原检测方法,认为RIHA法快速、灵敏、特异、简便。更多还原

【Abstract】 Calf diarrhoea, a common disease in the north of Huanghe River in both Anhui and Henan provinces since 1981, has been etiologically unknown. The present study demonstrates it to be a rotaviral diseazse, with a detection rate of 48.33% by TEM. Of 105 excrement samples, 34 wese detected as positive by RIHA with detection rate at 37.1%. Typical rotavirus nucleic acid electrophorsis atlas were observed among 23 of 48 excrement samples by PAGEThe coincidencerate of the 2 methods was 91.7%. Rotavirus antibodies were found ni 182 of 210 female cattle serum Samples by RIHI. Four neonatal calves which were nioculated with cattle rotavirus adapted in cell through mouth niduced clinical symptoms from 41.5 hours after nioculation. The cure rates were 95.27%, and 91.89% respectively, by experimental catttle gamma globulin and cattle serum interferon treatments.更多还原