【摘要】 应用流式细胞仪对46例大肠癌细胞进行DNA含量测定、增殖活性分析并研究二者病理形态学及预后的关系。结果表明:大肠癌细胞DNA指数(DI)为0.81—1.97,非整倍体占76.1％,平均增殖指数(PI)为33.85％。PI随核分裂象增多而逐渐增大,二倍体肿瘤多分化较好,呈团块状生长且患者生存期较长。去除粘液腺癌和印戒细胞癌后发现:DI和PI呈正相关关系,后者在二倍体及团缺状生长的肿瘤较低。因此,DI和PI可作为判断大肠癌恶性度的重要参考指标,并为反映癌生物学行为的组织学分型、核分裂象、生长方式和预后估计提供细胞生物依据。更多还原

【Abstract】 The cellular DNA content and proliferative activity of 46 colorectal carcinomas were measured by flow cytometry and its relationship to pathomorphology as well as to prognosis was analysed . The results showed that the DNA Index (DI) ranged from 0.81 to 1.97. The aneuploid tumor was 76.1%. Proliferative Index (PI) was 33.85%. PI increased with the mitotic activity. Most of the well differentiated adenocarcinomas showing massive growth pattern and with better prognosis were diploid . If the mucinous adenocarcinomas and signet-ring cell carcinomas were excluded, DI was correlated with PI, and the PI was lower in the diploid tumors which showed massive growth pattern. Both DI and PI may be considered as indicators of maHgnacy and these indicators presented the cytpbiologic base of histologic typing growth pattern and mitotic activity which reflected the invasiveness of carcinomas and fac-ptors for predicting prognosis.更多还原