【摘要】 <正> 霍乱原B亚单位(choleragen subunit B,CB)可和神经元胞体或突起膜上的特异性受点结合,进而经受体介导性内吞作用入胞,受体-配体复合物可逆行从轴突终末转运至胞体及树突或顺行从胞体至轴突终末。万选才等将CB与辣根过氧化物酶(horseradish peroxidase。HRP)经双功能试剂共价桥连,生产的CB-HRP复合物用于神经追踪,其敏感性较普通HRP提高了约130倍,发现了用以往任何更多还原

【Abstract】 Non-toxic choleragen subunit B (CB) was used as an antigenic neuronal tracer , Stained immunocytochemically with a mixture of two clones of anti-CB monoclonal antibodies and mouse peroxidase anti-peroxidase complex (PAP), CB can be utilized in both peripheral and central nervous systems for anterograde and retrograde neuronal tracings. It can offer not only high sensitivity and specificity, but also Golgi-like immunoperoxidase staining of labelled neurons.更多还原