【摘要】 非放射性标记探针的应用,对于推广基因诊断技术有重要意义。本文研究酶促和光促两法进行生物素标记DNA。以碱性磷酸酶偶联体系检测两法得到的生物素探针及其分子杂交产物,显色灵敏度近1pg,杂交灵敏度达5pg,而5～10×10~5倍的无关DNA没有或痕量非特异性显色。表明此两种生物素标记探针以及所使用的分子杂交和显色体系皆可用于病毒或真核基因的分析和检测。更多还原

【Abstract】 Nonradioactive probes in nucleic acid hybridization are important for popularization of gene diagnosis techniques. In this paper, enzymatic and ph?otometic methods for labeling DNA with biotin were studied. The colorime-tric sensitivity of these biotinylated probes is about 1pg and that of hybridization one, 5pg, while 5-10×105 folds of unrelated DNA show no signal. It is suggested that the two biotinylated DNA probes and system for hybridization and colorimetric detection could be used in gene analysis of virus and human genome.更多还原