【摘要】 用人浆细胞瘤细胞与小鼠网织红细胞异种杂交获得成功。杂交细胞可以传代,出现下列明显的肿瘤细胞去恶性化特征:①生长速度及~H-TdR并合率明显下降;②出现红细胞特异的遗传标记——血红蛋白;③出现慢型迁移率的葡萄糖-6-磷酸异构酶Gpi;④在软琼脂培养基中不形成细胞集落;⑤异种移植至裸鼠后丧失发生肿瘤的能力。表明红细胞胞质体杂交模型可以用作研究细胞恶性调控的实验系统。对调控肿瘤细胞恶性表型的可能机理进行了讨论。更多还原

【Abstract】 The present paper reported a heterologous cybrid cell line established in our laboratory by fusion of mouse reliculocytes with human plasmocy-toma cells. These cybrid cells could be continuously cultured and passaged without loss of characteristics of decancerization : 1. Obvious decrease of growth rate and incorporation rate of 3H-thymidine in nuclei; 2. Expression of specific genetic marker of reticulocyte hemoglobin; 3. Expression of glucose-6-phosphate isomerase with low mobility rate; 4. Loss of growth ability in soft-agar medium; and 5. Nontumorigenicity under heterotransplantation in to nude mice. These results provided further evidence, confirming this erythroid cy-brid model being useful in studies on malignancy regulation. The possible mechanism of cybrid model in regulation of tumor malignant phenotype was discussed.更多还原