【摘要】 以百合小鳞茎离体发生为实验系统,研究了鳞片(外植体)内多胺和多胺氧化酶活性的变化与小鳞茎发生之间的相关性。随着细胞脱分化、愈伤组织形成、小鳞茎发生等过程,内源腐胺、亚精胺和精胺的含量均失上升后下降,变化曲线为“钟形”,其含量达最高峰的时间为肉眼可见小鳞茎出现之前。多胺氧化酶活性在小鳞茎发生初期逐渐下降,6d后开始上升,并在小鳞茎突起时达最大值。由于内源多胺水平和多胺氧化酶活性的变化与小鳞茎发生有密切关系,可作为这一形态发生过程的生化指示。更多还原

【Abstract】 The relationship between polyamine me-tabolism and morphogenesis of bulblets frombulb scales of Lilium davidii var. unicolor invitro has been studied. It is noted that the pat-terns of change of the concentration of threepolyamine (putrescine, spermiddine and sper-mine) during the morphogenesis of bulblets aresimilar. Because the rates of change of threepolyamine concentration are different, theratio of putrescine to the sum of spermidine andspermine declines during the morphogenesisof bulblets. Although it has been reported that thepolyamine oxidase (PAO) is not involved inprocesses controlling senescence in oat leaves,the results obtained in our experiments indicatethat the change of PAO activity is related tothe morphogenesis of bulblets. Thus, polyamine concentration and PAOactivity may serve as biochemical markers forthe morphogenesis of bu?blets from bulb scalesin vitro.更多还原