【摘要】 采用一种简便而快速的方法分离了盐泽螺旋藻的藻胆体。藻胆体的最大吸收波长位于618 nm,室温下荧光反射峰位于677～678 nm。利用7～15％SDS—聚丙烯酸胺梯度凝胶板状电泳,可分出三条有色多肽,其中藻蓝蛋白的α亚单位与别藻蓝蛋白的α亚单位几乎重叠,不易区分;另有分子量为117,99,53,49,27,24.5和14kD的七条无色多肽。117和 99kD多肽可能联结藻胆体和类囊体,并作为末端能量受体,而14kD多肽多为“核”亚结构的组分,其余的可能为“棒”亚结构内和“核”“棒”亚结构间的联结蛋白。更多还原

【Abstract】 Phycobillisomes of Spirulina subsalsa wereseparated using a simple method in stead oftime-consuming sucrose linear gradient super-centrifugation and characterized by its ab--sorption spectrum, fluorescence spectrum andpolypeptide profiles. The room-temperatureabsorption spectrum showed the maximum at618 nm and a shoulder at 650 nm and the fluo-rescence spectrum at room temperature ex-hibited the maximum at 677～678nm. More-over, ten polypeptides were resolved from thephycobillisome of S. subsalas by 7～15% sodiumdodecyl sulfate gradient polyacrylamide slabgel electrophoresis. Three of them werecoloured and associated with phycobilins (phy-cocyanin and allophycyanin chromophores),and the others were uncoloured polypeptidewhich were visible only after the gels werestained with Coomassie Bri1liant Blue G-250. The larger polypeptides, 117 and 99 kD, porbably served to attach the phycobillisomes tothylakoid membrane and acted as a terminalenergy acceptor and the 14 kD polypeptidemight belong to the ’core substructure com-ponent. We inferred that the other linker poly-peptides should be responsible for "rod" sub-structure of phycobillisome. These resultsindicate that the spectroscopic properties andpolypeptide composition of phycobillisome fromS. subsalsa are similar to those reported for otherblue-green algae and that the phycobillisomefrom S. subsalsa obtained by the simple meth-od can also retain its integrity of structure andenergy stransfer.更多还原