【摘要】 用高分辨率的蛋白质双向电泳对人肝癌及正常肝组织的胞浆蛋白质进行检测。结果表明肝细胞癌变后,胞浆蛋白质双向电泳图谱发生了显著改变,总点数增加,蛋白质点的分布向低分子量低等电点偏移,出现了一些新的蛋白质点,有些蛋白质点密度增加,有些蛋白质点密度减少或消失。更多还原

【Abstract】 Cytosoi proteins were prepared from human hepatoma and normal liver tissues respectively obtained from surgery. Two-dimensional gel electrophoresis with silver staim-i;] was used to study protein pattern. The molecular weight ranged fron 100,000 and the isoele-ctric points ranged from pH 4.65 to 7.34. The gels were diagramea into ; areas according to molecular weight and isoelectric point and alphabetized from A to I. The protein spots were counted, and the spot densities were compared between hepatoma and normal liver tissue. The results indicated that there was a significant difference of protein patterns in 2-D gel electrophoresis between hepatoma and normal liver tissues. 686 spots in hepatoma and 646 spots in normal liver tissues were identified. But the protein spots of hepatona tended to distribute at relatively lower molecular weight and lower isoelectric point areas. Among the striking spots, 27 spots and 2 clusters of spots in hepatoma were not noted in normal liver tissues; 13 spots and 7 clusters of spots in hepatoma had higher densities than those in normal liver tissues; 19 spots and 2 clusters of spots in normal liver tissues were not noted in hepatoma; 12 spots and 10 clusters of spots in hepatoma had lower densities than those in normal liver tissues.更多还原