【摘要】 作者对基因工程转化细胞株MT-5的HBsAg表达特性进行了观察分析。结果表明:细胞的连续传代对HBsAg的表达稳定性没有影响;MT-5的不同单细胞亚克隆株中的HBsAg表达量存在不均一性;HBsAg的表达不但受重金属离子的诱导,而且细胞可以耐受较高浓度ZnCl₂的处理;MT-5细胞表达的HBsAg具有糖基化多肽,是制备疫苗的理想材料;通过培养条件最优化,筛选高产亚克隆细胞株和采用最适重金属离子诱导浓度,HBsAg的产量达到2.5mg/1,即2.2×10^-12g/细胞。更多还原

【Abstract】 We observed and analysed the expression characteristics of HBsAg in the transformed cell line MT-5. The results revealed that the expression and excretion of HBsAg is stable; the cells could stand 200μm ZnCl, and the yield of HBsAg could be induced by ZnCl2 up to 2.5mg/l, we established several subcloned cell lines of MT-5 and The expression yield of HBsAg is different in different sudline HBsAg expressed by MT-5 showed 23K and 27K polypeptides by SDS-PAGE analysis. It is evident that the HBsAg can be glycosylated in MT-5 cells.更多还原