【摘要】 本病是我国新发现的一种家兔烈性传染病,对青、成年兔具有高度发病率和致死率。已从病兔组织中成功地分离到病毒。经蔗糖密度梯度和氯化铯等密度离心纯化的病毒出现两个峰。两峰病毒均为球形、无囊膜,且均有两种病毒颗粒—完整的病毒颗粒（分别约占80%和60%）和空心病毒颗粒（分别约占20%和40%）。第一峰病毒在氯化铯中的浮密度为1.28—1.34克/毫升;第二峰病毒浮密度为1.36-1.44克/毫升,沉降系数为85_s。用氯仿处理和冻融的方法澄清兔瘟肝组织液,再经PEG沉淀、氯仿处理和Sepharose 4B柱层析,得到纯化病毒制剂。该制剂能致家兔发生典型兔瘟。电镜观察,病毒粒子无囊膜,直经32-34nm,蕊髓直径15-17nm,衣壳厚8-9nm,呈特征的20面体T=3“格子样”表面对称,壳粒数为32,亮粒直径为8-10nm,高4nm,包括12个五邻体和20个六邻体,共180个原体,原体直经约4nm。此外,还见有少数空心衣壳和直经为23-27nm的“病毒样颗粒”。纯化病毒制剂煮沸裂解后径SDS电泳,与未经裂解的样品对照,发现病毒粒子具有3条结构多肽,分子量分别为Vp₁ 76000、Vp₂ 62000和Vp₃ 52000,Vp₂为主要多肽。本病毒具有抗酸性,在1克分子氯化镁溶液中对抗的热有稳定的作用。能凝集人类O型红血球,而不能凝集其他动物（包括兔）的红血球。血球凝集能被特异抗血清?更多还原

【Abstract】 Rabbit pest is a newly found virus infection in rabbits ir China, causing high mortality and beavy losses. The viruses were isolated successfully from infected rabbit tissues and purified by sacrose-velocity-gradient and ecsium-chloride-equilibrum ultracen trifugation and after that two virus peaks appeared. The virus particles in the two peaks had a spherical shape no envelopes and two different morphological virions: the full virions （80% and 20% respectively） and the empty virions （20% and 40% resp）.The particles in the first peak had a buoyant density of 1.28-1.34g/ml in cesium chioride. Those in the second peak had a buoyant density of 1.36-1.44g/ml and a sedimentation coefficient of 85s. The liver extract of diseased rabbit was clarified by means of chloroform treatment, and freezing and thowing. The clarified extract was further purified by Sepharose 4B column chromatagraphy after PEG precipitation and chroroform treatment The purified RPeV could cause typical Rabbit pest.It was shown under the EM that tne purified viruses were nonenveloped particles, 32-34 nm in diameter, core measure 15-17 nm, with icosahedrul T=3 surface lattice sym metry, 32 capsomeres, 8-10 nm in diameter, 4 nm in height, including 12 pentamers and 20 hexamers, 180 subunits, 4 nm in diameter. In addition, a few of empty particles and virus-like particles （23-27 nm in diameter） were observed. By SDS-PAGE of the purified material, compared with non-disintergrated control, it was demonstrated that the virus particles contained three structural polypeptides, with molecular weight of VP₁ 76000, VP₂ 62000 and VP₃ 52000. The main polypeptide was Vp₂. The virus was acidfast and stable in the solution of 1M MgCl₂, and could agglutinate the O type of human red blood cells, but not that of the other animals （including rabbit red blood cells）. The hemagglutination could be inhibited by specific antiserum. The LD50 of the diseased rabbit liver tissues to rabbits was 10^-7 10^-7.5.The viruses collected from different places were shown to have identical serotype by cross-protection in rabbits, immunomicruscope, he magglutination and hemagglutination inhibitory test. The viruses preserved in-20℃ for 18 months did not loss their infectivity. The classification of the virus was unknown since no tissue cultures （primary and cell lines） fit for replication of the virus were as yet. The tissue inactivated vaccine for the disease was available and had been proved to be excellent due to its high level of protection and easy distribution,storage and administration. A significant level of protection was developed within a week and the protection （?）asted for eight months. Vaccination of twice a year could control the disease effectively.更多还原