【摘要】 采用推理选育技术对杆菌肽产生菌Bacillus lichetnformis 选育,其效果明显优于随机选育.从那些在分别含有高浓度FeSO₄、泛酸、钒酸盐、杆菌肽、异亮氨酸或疏脲的培养基中处于优势生长或产生较大抑菌圈的去调节菌株中得到一些杆菌肽高产菌株.杆菌肽产量从出发菌株86-10的197u/ml到突变株Bc-6的543u/CI,效价提高176%.更多还原

【Abstract】 Conventional strain and medium improvement techniques were of limited success in increasing yields of the antibiotic bacitracin. High-yield strains were obtained by selections of deregulated mutant which grow quickly on media containing high concentrations of FeSO4, pantothenid acid, vanadate, bacitracin, isolucine, and thiourea, respectively. Large inhibition zones resulted in presence of those compounds at high concentrations. Some of these mutants have higher yield of bacitracin than that of wild type.更多还原