【摘要】 应用细胞电泳技术,对两分离株溶组织内阿米巴进行观察。结果:在pH2.05～8.00梯度的McIlvaine缓冲液中的细胞电泳表明,当pH3.00～8.00时,电泳率(EPM)为负值,细胞表面带负电荷。在除pH2.05外的其他pH梯度中,两分离株阿米巴的EPM有明显差别。A、B两分离株阿米巴的表面电荷密度分别为:148.3～-6035.9esu/cm~2和222,4～-6624,Oesu/cm~2。酶实验表明:两分离株阿米巴表面无唾液酸,其表面负电荷基团对胰蛋白酶和脱氧核糖核酸酶Ⅰ敏感。更多还原

【Abstract】 In this paper, the variation of surface electricity of two Entamoeba histolytica isolar es was investigated using the cell electrophoretic technique. By testing the electrophoretic iondition of amoeba in gradient pH and the effect of some enzymes, we provided infor-nations for the physiology and pathogenicity of this parasite.Cell electrophoresis for the two isolates of E. histolytica (isolate A and isolate B) n pH 2.05-8.00 Mcllvaine buffer revealed that EPM (electrophoretic mobility) was negative under the pH gradients of 3.00-8.00, which meant the amoeba cell surfacecar- ried negative charge in this condition. Surface charge densities of isolates A and B were found to be ( + ) 148.3-(-) 6035.9 esu/cm2 and (+ )222.4-(-) 6624.0 esu/cm2 respectively. In addition, except in pH 2.05, the EPMs in Mcllvaine buffer were different between the two amoeba isolates, with significant statistical difference.The enzyme test demonstrated there was no neuraminic acid on the surface of these two amoeba isolates. The surface negative groups of the protozoa were sensitive to trypsin and deoxyribonuclease I.更多还原