【摘要】 用硫酸铵分段沉淀法将猪囊尾蚴及其囊液的蛋白质分别分离为8种抗原,用间接酶标法检测猪囊尾蚴病病人、其他寄生虫病人及正常人血清中的相应抗体。结果表明,在8种抗原制品中,囊液部分提纯抗原P₁诊断囊尾蚴病的阳性率最高。囊尾蚴粗抗原的部分提纯成份P_25-55的阳性率次之。 P₁和囊液粗抗原对包虫病病人血清存在严重的交叉反应,而P_25-55交叉反应较低。P₁与肺吸虫病病人血清有交叉反应,但P_25-55与肺吸虫病人血清无交叉反应。P₂5-55的来源比P₁丰富,是此病免疫诊断的实用抗原。更多还原

【Abstract】 The proteins of cysticercus cyst fluid and cysticercus were fractionated into 2 and 4 fractions respectively by ammonium sulfate precipitation. ELISA tests were performed by using these 8 preparations (2 crude preparations and their 6 fractions) as antigens tc determine the presence of corresponding antibodies in the sera of proved cases of cysticercosis and other parasitoses, as well as normal adults. Our study shows that a fraction of cysticercus cyst fluid, P1, has the highest positive rates in the diagnosis of cysticercosis and OD ratios of cysticercosis patients to normal persons among 8 preparations. Our study also shows that the positive rate of a fraction of crude cysticercus preparation, P25-55, is only next to P1 among 8 preparations and has highest OD ratios among crude cysticercus preparation and its fractions.With sera of hydatidosis patients, P1 is highly cross reactive, while P25-55 is less so cross-reactive. With sera of paragonimiasis patients, P1 is also cross-reactive, while P25-55 is not in 14 patients studied.In addition, there is a richer source of P25-55 than PI. Therefore, P25-55 is practically a good antigen fraction for diagnostic purpose,更多还原