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五个地理株周期型马来丝虫成虫蛋白和同工酶电泳分析

ELECTROPHORETIC ANALYSIS ON PROTEIN AND ISOENZYME PATTERNS OF FIVE GEOGRAPHICAL STRAINS OF ADULT PERIODIC BRUGIA MALAYI

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【作者】 黄蕙芬郑福申李杰张丕哲左迅叶淑铭聂文清

【Author】 Huang Huifen, Zheng Fushen, Li Jie, Zhang Pizhe, Zuo Xun Advisor: Ye Shuming, Nie Wenqing Department of Paras itology, Dalian Medical College

【机构】 大连医学院寄生虫学教研室大连医学院寄生虫学教研室

【摘要】 本文应用圆盘电泳对五个地理株——福建建阳株、安徽泾县株、四川乐山株、贵州独山株和贵州荔波株周期型马来丝虫成虫的蛋白和七种酶进行了区带分析和比较。结果显示五个虫株的蛋白、PGM和PO同工酶电泳型没有差异;而MDH和GPI的同工酶电泳型出现了两种类型,即贵州省内的两个株与另外三个株不同;G6PD、EsT和LDH没有区带出现。

【Abstract】 Protein and isoenzyme electrophoretic patterns of phos phoglucomutase (PGM), glucose phosphate isomerase(GPI), glucose-6-phosphate dehydrogenase(G6PD), malate dehydrogenase(MDH), esterase(EST), lactate dehydrogenase(LDH) and peroxidase(PO) of five geographical strains (Jianyang Fujian Strain, Jingxian Anhui Strain, Leshan Sichuan Strain, Dushan Guizhou Strain and Libo Guizhou Strain)of adult periodic Brugia malayi were comparatively analysed by disc electrophoresis. No significant difference in protein and isoenzyme patterns of PGM and PO was observed among the five geographical strains. On the other hand, both MDH and GPI showed two different isoenzyme patterns, i. e, there existed obvious difference in MDH and GPI isoenzyme patterns between the two Guizhou strains and the other three ones. No band was found in the isoenzyme patterns of G6PD, EST and LDH.

  • 【文献出处】 中国寄生虫病防治杂志 ,Chinese Journal of Parasitic Disease Control , 编辑部邮箱 ,1988年01期
  • 【被引频次】8
  • 【下载频次】7
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