【摘要】 本文详细报道一个高度灵敏、特异、准确和稳定的血清生长介素(SMC)放射免疫测定方法。以50例中国正常成人的混合血清作参考标准,其SMC浓度定为1kU/L。方法的最小可测量为0.004±0.003kU/L,批内变异为7.8±1.1％,批间变异为13.6±2.5％。肢端肥大症病人血清的稀释曲线与参考标准曲线和SMC标准曲线呈很好的平行关系。74例正常成人血清SMC范围为0.38～2.60 kU/L,17例肢端肥大病人为4.20～26.00kU/L,与正常成人不重叠。本文还对影响方法稳定性的因素进行了研究。更多还原

【Abstract】 This article described a sensitive, specific, accurate and stable radioimmunoassay for serum somatornedin C(SMC). A serum pool of 50 Chinese normal adults was used as reference standard. The minimal and its concentration was assigned as 1kU/L and detectable level was 0.004 + 0.003 kU/L. The coefficient of variations were 7.8 +1. 1% for intra-assay and 13.4 + 2.5% for inter-assay. The dilution curve of the acromegalic serum paralleled to those of the reference standard and SMC standard. The concentration of serum SMC in 74 normal adults ranged from 0.38 to 2.60kU/L. The range of serum SMC in 17 acromegaly patients was 4.20-26. 00kU/L which was significantly higher than that of the normal adults (P<0.001). The factors influencing the stability of this RIA were also studied.更多还原