【摘要】 作者分别从小牛脾脏和猪血浆中提取了粒细胞生成抑制因子(GIF)和血浆非特异性免疫抑制蛋白(PNIP),并对它们的生物学活性进行检测。GIF能够明显抑制BaBL/C小鼠骨髓粒-巨噬细胞集落形成单位(GM-CFU),但对小鼠脾脏细胞及小鼠淋巴瘤L5178Y细胞的~3H-TdR掺入无明显作用;PNIP能够显著地抑制C57BL/6J小鼠脾脏和人外周血淋巴细胞的增殖反应,同时也能够抑制小鼠淋巴瘤L5178Y细胞和人淋巴瘤Raji细胞的生长,但对非淋巴细胞系列的Hela细胞和Vero-E6细胞则无明显作用。GIF和PNIP均可使相应靶细胞的辐射敏感性降低。经GIF处理的骨髓细胞,受照射后其GM-CFU较对照组明显增多;经PNIP处理的L5178Y细胞受照射后其细胞生存率和~3H-TdR掺入率亦均较对照组为高。组织抑制因子的辐射防护作用与它们作用于靶细胞的时间和浓度以及照射剂量的大小密切相关。更多还原

【Abstract】 Two inhibitory substances were isolated from the calf spleens and the porcine blood, which were respectively designated to as Granulopoiesis Inhibitory Factor (GIF) and Plasma Nonspecific Immunosuppressive Protein (PNIP).The biological activity of GIF and PNIP was examined.GIF could remarkably inhibit GM-CFU of the BaBL/C mouse bone marrow cells but had no effect on the 3H-TdR incorporation into the mouse spleen cells and mouse lymphoma L5178Y cells.PNIP could significantly suppress the proliferation of the lymphocytes from human peripheral blood and C57BL/6J mouse spleen and the malignant lymphoid cells,L5178Y cells and Raji cells, but had no effect on Hela cells and Vero-E6 cells which are non?lymphoid cells.GIF and PNIP could also result in the decline of the radiosensi-tivity of their responsive target cells to e(1Co Gamma rays .When the GIF-treated mouse bone marrow cells were irradiated,the number of their GM-CFU was much more than that of the control.The survival rate and the 3H-TdR incorporational rate of the L5178Y cells which were exposure to PNIP and then were irradiated was higher than that of the control.The radioprotective activity of the tissue inhibitory factors was closely related to the time of acting on the target cells, the concentration of them and the doses of irradiation.更多还原