【摘要】 <正> pH梯度薄层板目前已用于生物碱及有机酸等的分离,国外采用特制的涂铺器制备。本文采用两次展开法,制得的pH梯度薄层板的pH值递变范围与文献相似。用pH更多还原

【Abstract】 The pH gradient silica gel layer was prepared by twice developing method, and n-butanol-95% ethanolwater(84:11:5) was used as developing solvent. Thymol blue, methyl red and neutral red were used to determineits character. The cholic acid in Zaocys dhumnadcs (Cantor) was separated with pH gradient layer. On neutral layer chloroform-methanol-water(65:25:2) was used as developing solvent. The R value of cholic acid was 0.64. Determination of cholic acid was performed by using a Shimadzu CS-910 TLC scanner(λ_S=550nm, λ_R=700nm). The average recovery of cholic acid in Zaocys dhumnadcs(Cantor) was 100.9% (n=7) with a coefficient variation of 3.1%.更多还原