【摘要】 本文建立了测定炎痛喜康血药浓度的高效液相色谱法。酸化的血浆用乙醚5ml提取,以1-(2-吡啶偶氮)-2-萘酚为内标,提取溶剂吹干后用甲醇溶解进样。所用固定相为多孔硅胶-氰基(10μm),流动相为pH3的甲醇-0.1mol/L磷酸二氢钾(45∶55)。本法测得样品的最低检测限为2ng;最低检测浓度为50ng/ml(S/N>2),线性范围为0.2～10μg/ml。方法的平均相对回收率为99.5%,日内与日间精密度的变异系数均小于5%。更多还原

【Abstract】 Piroxicam involved in plasma was determined by HPLC. The acidified plasma （pH=4） was extracted by using diethyl ether, with l-（2-pyridylazo）-2-naphthol as an internal standard. The organic extract was reduced to dryness, the resultant residue was redissolved in methanol, and an aliquot of this solution was then chromatographed on a cyano-column, using a mobile phase of CH₃OH-0.1mol/LKH₂PO₄ （45:55） pH3.0. The sensitivity of the method was 0.05μg/ml of plasma level. The linearity was demonstrated over the range of 0.2～10μg/ml plasma with a correlation coefficient of 0.9997. The average recovery was 99.5%. The CV of within-day and day-to-day were all less than 5.0%.更多还原