【摘要】 用简易二步高压液相色谱法（HPLC）分析婴儿食品（强化食品）中维生素D₂的方法,系采用样品直接皂化,用适当的溶剂——苯,萃取不能皂化的物质,然后,进行第一步制备（纯化）高压液相色谱,用Nucleosil 5 C₁₈为固定相,以甲醇-乙腈（3:2）为流动相,作反相分离,除去大部分干扰物质,收集维生素D₂组分。此收集组分再进行第二步分析高压液相色谱,用Zorbax SIL为固定相,以异丙醇-正己烷（0.8:99.2）为流动相,作正相分离,使维生素D₂与其它伴随物分开,利用紫外吸收特性进行检测。样品的维生素D₂峰高与以同样条件下得到的维生素D₂标准品峰高比较后定量。其添加回收率在84％以上,重现性试验,其标准偏差为±12.57,变异系数为±4.57％。该法快速、准确,具有对婴儿食品分析的适用性。更多还原

【Abstract】 An improved method for the determination of Vitamin D2 in baby food (fortified dried milk) was established by using two steps of high-performance liquid chromatography (HPLC). The sample was directly saponified and extracted with benzene. The extracted unsaponifiable matter was firstly subjected to preparative HPLC using a Nucleosil 5C18 column (reversed-phase type) with acetonitrile-methanol (3:2) as a mobile phase. This first step was for the purpose of clean-up, and a fraction containing Vitamin D2 was collected. The separate fraction was subsequently subjected to analytic HPLC with a Zorbax SIL column (straight-phase type) with isopropanol-hexane (0.8 : 99.2) as a mobile phase. The Vitamin D2 was separated from other contaminants and determined with ultraviolet spectrograph by the peak height estimated. The recovery rate was 84%, standard deviation 12.57 and coefficient of variation 4.57%. The proposed method was rapid, accurate, and suitable for the determination of baby food.更多还原