【摘要】 本试验通过热处理、分生组织培养和花药培养进行脱毒研究。花药培养经过预先冷冻处理获得较高的出愈率及较强的再生能力。花药染色体镜检绝大部分与母株的染色体数相同,为2n=28,花药植株当年开花结果率达30％,可作为获得脱毒材料的方法之一。用热处理及分生组织培养的苗已在田间生长两年,总苗数5万株以上。花药植株于1987年5月、8月分别定植于田间4000株左右。更多还原

【Abstract】 The traditional production of strawberry plant face many difficulties, the grower is therefore obliged to grow runners himself, and this threatens their health. Experiments were undertaken to study heat treatment and meristem culture and anther culture for Virus-free straw-berry plants from traditional production of strawberry plant.The chilling treatment applied to the flowers before the excision of anthers highly increase the growth of callus and it’s potential for rege-neration. Most of the plants are diploid 2n = 28. The adaptation of the cultural strawberry plants to normal growing conditions was very simple. Transplantation of the young plantlet was 95% up successful.We obtained 50,000 in vitro plant by cutting the small axillary (0.3mm) buds fromed upon crown disk sliced from heat-treated plants.The plantlets from anther culture 4,000 plants were grown to the field in May and August of 1987.更多还原