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人参果中水溶性多糖的研究:杂多糖F的分离提纯与结构分析

STUDIES ON THE WATER SOLUBLE POLYSACCHARIDE FROM PANAX GINSENG FRUIT ISOLATION,PURIFICATION AND STRUCTURAL STUDIES ON HETEROGLYCAN F

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【作者】 刘春兰张翼伸李润秋

【Author】 CL Liu, YS Zhang and RQ Li (Department of Biology, Northeast Normal University, Changchun)

【机构】 东北师范大学生物系东北师范大学生物系 长春长春长春

【摘要】 <正> 人参根、茎、叶多糖的研究已有报道,人参果胶是人参根多糖中的有效成分,人参果多糖的研究尚未见报道。采用吉林省集安制药厂提取人参果皂甙后的残渣为原料,提取水溶性人参果多糖。人参果渣用热水提取,醇析后获褐色粗多糖。经纸层析、气相色谱分析,该多糖含阿拉伯糖、鼠李糖、木糖、葡萄糖、半乳糖及半乳糖醛酸六种单糖残基,含蛋白质6.25%。将粗多

【Abstract】 Polysaccharides extracted from ginseng fruit with water are a mixture of heteroglycans composed of 6 monosugars. A homogeneous heteroglycan F obtained by fractionation and purification contains Ara., Rha., Xyl., GalA., Gal., Glc., in the molar ratio of 5.9: 4.3: 0.3: 0.7: 17.4: 1.0. Its mol. wt. was shown to be about 1,900,000.The purity of F was established by ultracentrifugal, sepharose 4 B 2 B column chromatography, cellulose acetate film electrophoresis and polyacrylamide gel electrophoresis.By means of partial hydrolysis with acid or pectinase, periodate oxidation, Smith degradation, methylation analysis, GC and GC-MS, the structure of F has been identified as a highly branched polysaccharide with β-(1-3)-galactose linkages as its main chain and the side chains on the main chain at C4 and C6 positions.

【关键词】 人参人参果人参果多糖
【Key words】 Panax ginseng C. A. MeyGinseng fruitpolysaccharide
  • 【文献出处】 药学学报 ,Acta Pharmaceutica Sinica , 编辑部邮箱 ,1988年11期
  • 【被引频次】41
  • 【下载频次】290
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