【摘要】 我们研究了水稻珍汕97不育系和保持系的线粒体DNA的PstⅠ、HindⅢ、BamHⅠ酶切电泳带型,发现了不育系和保持系的线粒体DNA分子结构的显著不同,而不育系和保持系的叶绿体DNA的HindⅢ酶切片段没有差异。以线粒体的细胞色素C氧化酶亚基Ⅰ、Ⅱ(COⅠ、COⅡ)基因为探针,与珍汕97不育系、保持系的叶绿体、线粒体DNA酶切片段进行分子杂交,证明叶绿体DNA没有COⅠ、COⅡ基因的同源顺序,发现不育系和保持系COⅠ、COⅡ基因有组织结构上的差异,但不能确证这些差异是否与细胞质雄性不育有关。更多还原

【Abstract】 We analysed the mtDNAs (mitochondrial DNAs) and ctDNAs (chloroplast DNAs) isolated from fertile (Zhenshan 97B) and male-sterile (Zhenshan 97A) cytoplasms by using restriction endonuclease Pal,HdIII and BamHl.The results showed that mtDNAs from Zhenshan 97A and 97B cytoplasms were distinguishable.Southern hybridization,using labelled mt genes (cytochrome C oxidase subunit I,II,COI,COII) of rice,indicated that no presence of mtDNA-homologous region on ctDNA fragments and the structural difference of COI,COII genes between fertile and male-sterile cytoplasm line.It has not been demonstrated whether different mtDNA of normal and male-sterile cytoplasm of rice is associated with cytoplas-mic male sterility.更多还原