【摘要】 利用离体非做功的大鼠心模拟缺血和再灌注,通过对琥珀酸脱氢酶进行组织化学染色以显示损伤范围和程度,探讨了早期显现再灌注损伤的方法以及肌原纤维损伤与脱氢酶缺失的关系。结果表明,心肌缺血30min再灌注30min出现该酶活性降低,形成再灌注损伤。组化染色与光镜对比观察证明,脱氢酶缺失区与心肌损伤区的部位和范围基本一致,提示此方法适用于再灌注损伤的研究。更多还原

【Abstract】 Histochemical stain of succino-dehydrogenas in myocardium was made in non-working rat hearts to show the range anddegree of injury by st of ischemia and reperfusion after ischemia and investigated the method with which the reperfusion injury was earlyshown. The result showed that myocardium of reperfusion for 30 min after ischemia for 30 mim could reduce the activity of succino-dehydrogenase and form reperfusion injury.It is proved that the district of dehydrogenase loss was basically consistent with the district of myocardium injury whether in position or in range by contrast the histochemcial stain with light microscope.更多还原