【摘要】 本文对酸性α-醋酸荼酯酶标记人T细胞染色法的影响因素作了研究,并对其操作程序作了改进。改进方法对正常人和肿瘤患者外周血中淋巴细胞进行估价,结果为正常组ANAE阳性率占70.90±1.93％,肺癌组占47.08±8.90％,消化道肿瘤组占45.20±5.57％。正常组和肿瘤组之间的差异有非常显著性(P<0.01)。更多还原

【Abstract】 The influencial factors of the Acid alpha -Naphthyl Acetate Esterase (ANAE) stain method for human T lymphocytes were studied and some modifications were made to the procedures ,such as the blood smear preparation, incubation solution concentration, length of incubation period and washing time, etc. The established method was employed to assess the ANAE activity in lymphocytes in peripheral blood of normal individuals and cancer patients. The results showed that positive ANAE accounted for 70.90 ±1.93% in normalgroup ( n=10 ) , 47.08±8.90% in lung cancer group (n=6 ), and 45.20±5.57% in digestive tract cancer group (n = 10). The differences between the normal and the cancer groups are significant ( P<0.01 ) . Since it is simple and rather specific for T lymphocytes, the ANAE stain method can be used clinically as an accessary examination in the diagnosis of certain cancer cases.更多还原