【摘要】 利用DEAE-Sephadex和Phenyl-Sepharose层析,同时从猪脑中分离纯化了两种钙结合蛋白,即S-100和CaM。分子量为10,500的钙结合蛋白对牛脑PDE无激活作用;与CaM抗体无交叉反应;在SDS-PAGE中,迁移速度不受Ca²⁺的影响;而在碱性甘油PAGE中,迁移速度在有Ca²⁺时比无Ca²⁺时慢,并且出现多区带;在250、260、266、270、279和284nm处具有六个吸收峰。以上特征及其氨基酸组成分析表明,此蛋白为S—100。另一种钙结合蛋白分子量为18,000;对牛脑PDE的激活作用和电泳行为与标准CaM相同;与CaM抗体呈阳性反应。更多还原

【Abstract】 A Purification method of calmodulin and S-100 to homogeneities simultaneously from porcine brain is described. Two calcium-binding proteins, CaBP1 and CaBP2, have been isolated from porcine brain by DEAE-Sephadex and Phenyl-Sepharose chromatography. CaBP1 did not stimulate cyclic nuc-leotide phosphodiesterase and was eventually identifed as S-100 by its molecular weight, electrophoretic characterization, ultraviolet absorption spectra and amino acid composition. CaBP2 , on the other hand could stimulate cyclic nueleotide phosphodiesterase and react with anti-calmodulin sera and had a molecular weight and electrophoretic profiles which are identical to those of calmodulin.An obvious advantage of the present procedure over the previously reported methods of individual purification is to purify calmodulin and S-100 at the same time in a simple, economic and effective way, especially for large-scale purification.更多还原