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人红细胞的Ca2+和Cd2+运输
Ca2 + AND Cd2 + TRANSPORT IN HUMAN ERYTHROCYTE
【摘要】 用人红细胞膜、翻膜小囊和完整红细胞研究了Cd2+对Cd2+运输的影响与Cd2+-ATP酶活性的关系,以及细胞对Cd2+的摄取和影响摄取的因素。Cd2+抑制Cd2+运输,与抑制Cd2+-ATP酶具有相关性。Cd2+极易进入细胞,一旦进入,难于排出。进入细胞的Cd2+主要与胞浆和膜蛋白相结合。
【Abstract】 In this study, we investigated the effects of Cd2+ on the Ca2 + -ATPase of human erythrocyte membranes, the Ca2 + transport in inside-out vesicles, and the Cd2 + transport in intact erythrocytes. Cd2+ inhibited the Ca2 + -ATPase activity of human red blood cell membranes, probably through an effect on the catalytic site of the enzyme. The inhibition by Cd2+ could be reversed by thiols, such as dithiothretol and glutathionine. Cd2 + had an inhibitory effect on the Ca2 + uptake by inside-out membrane vesicles.This inhibitory effect correlated well with that on the Ca2 + -ATPase. In contrast with the marked impermeability to Ca2 + , the red cells were able to accumulate Cd2 + , which was accelerated by the calcium ionophore A 23187 and glucose. As most Cd ions entering the red cells were associated with cytosol and membrane bound proteins, the cations were released very slowly into the medium.
【Key words】 erythrocyte; inside-out vesicles; calcium; cadmium toxicity; Ca2+ -ATPase; calmodulin; human;
- 【文献出处】 徐州医学院学报 ,Acta Academiae Medicinae Xuzhou , 编辑部邮箱 ,1988年01期
- 【被引频次】14
- 【下载频次】35