【摘要】 用淋巴细胞杂交瘤技术得到了株抗E.coli K88抗原的特异单克隆抗体（MCA）:JLZK-7,-10和-178。小鼠腹水中MCA的免疫荧光滴度达10^-5～10^-6,直接凝集滴度为 1:500～1:5000,比用常规方法生产的因子血清高100～1000倍。JLZK-7为IgG₁亚类,JLZK-10为IgG₂₆亚类,JLZK-178为IgG₃亚类。这3株MCA与K88阴性大肠杆菌和肠杆菌科中的其他细菌无交叉反应。对K88阳性菌株的反应性测定表明,JLZK-7是对K88ab、K88ac和K88ad菌株都起反应的群特异MCA,JLZK-10和-178是分别仅与部分K88ab菌株和部分K88ac菌株反应的型特异MCA。试验结果表明,这3株MCA可用于初生仔猪下痢病的诊断和大肠杆菌K88血清型鉴定。更多还原

【Abstract】 Three hybridoma cell lines, JLZK-7, JLZK-10, JLZK-178, secreting specific antibodies to K88 adhesin antigens of enterotoxigenic E.coli from swine were derived from two separate fusions between mouse myeloma cell line SP2/0 and spleen cells from mice immunized with purified K88 adhesin antigens. The antibodies from culture supernatant had titers up to 100 in the indirect immunofluorescence test, and those from mouse ascitic fluid had titers ranging from 105-106. As shown in either immunofluorescence test or direct agglutination test,MCA JLZK-7 belonged to the type common, i.e.it reacted with all the K88 adhesin bearing E.coli strains tested, including K88ab, K88ac, or K88ad; MCAs JLZK-10 and JLZK-178,on the other hand, belonged to the type specific,i.e.they only reacted with K88ab or K88ac bearing E.coli strains. All the 3 MCAs did not react with E.coli strains bearing adhesins other than K88 E.coli strains without any adhesins,and other bacterial species in the fa-mily of Enterobacteriaceae.更多还原