【摘要】 本文报导用比色法测定蜂花粉中总黄酮含量的方法。此法采用70％乙醇提取,以5％亚硝酸纳溶液,10％硝酸铝溶液和氢氧化钠试液为显色剂,在波长510nm处测定吸收度。方法简便易行,重现性好,加样回收率为98.99％,变异系数1.29％。更多还原

【Abstract】 Quantitative determination of the flavonolds in Bee Pollen with colorimetry is described. Bee Pollen was extracted using 70% ethanol. The extract was developed by 5% sodium nitrite, 10% aluminum nitrate and sodium hydroxide test solution. The wavelength of absorbance Is 510 nm. The recoveries of Bee Pollen were 98.99% and the variation coefficients were 1.29%. The method is simple, sensitive and reproducible. It was applied to the determination of flavonoids from the Pollen of Helianthus annuus L., Zea Mays L., Fagopyrum esculentum Moench. and Brassica Campestris L. The results showed that the flavonoid contents in Pollen of Brassica campestris L. were higher than those in other Pollens.更多还原