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水稻与高粱杂种叶绿体类囊体膜蛋白质分析
THE CHLOROPLAST THYLAKOID MEMBRANE PROTEINS ANALYSIS IN HYBRIDS OF RICE WITH SORGHUM
【摘要】 本文采用 SDS—PAGE 方法,对水稻与高粱杂交后代进行了叶绿体类囊体膜蛋白组成成分的比较分析。所得结果表明,杂种中多数株系比母本对照水稻银坊多显示3条分子量分别为13.5kd、18kd 及64kd 的多肽带~*。其中18kd 为父本高粱所具有,另外两条带为母本水稻及父本高粱所不具有,而为杂种水稻所具有的。这就表明,父本高粱花粉在杂交中的作用以及所得杂种的新的叶绿体和核的基因组在杂种后代膜蛋白的形成中的作用是明显的。文中对三条多肽的性质与功能进行了讨论。
【Abstract】 In this experiment using the method of SDS-PAGE comparison andanalysis on the compositions of chloroplast thylakoid membrane proteinsof hybrid lines obtained from the hybridization of rice with sorghumpollen were performed.Results obtained indicated much more of linesin the progenies gave three polypeptides with molecular weights of 13.5kd,18 kd,and 64 kd respectively that were absent in rice materal Yinfang.Of which 18 kd polypeptide was observed only in the sorghumpaternal.The other two were revealed in hybrid lines but not possessedboth in materal and pateral.This means the significant role of the sorghum pollen and the new combination of chloroplast and naclear genomesof hybrids during the character formation in the hybrid generations.
【Key words】 Rice × Sorghum; Chloroplast thylakoid membrane protein; SDS-PAGE;
- 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,1988年02期
- 【被引频次】4
- 【下载频次】62