【摘要】 本文首次采用改良的人全血化学发光法检测了不同浓度硒对10例健康成人白细胞氧化活性的直接影响。将四种不同浓度硒分别加入健康成人新鲜抗凝全血中,使其最终浓度分别为0.001μg/ml、0.01μg/ml、0.1μg/ml和1μg/ml,4℃冰箱储存,并于1小时、12小时和36小时进行化学发光的动力学测量,其结果与加硒前相比,化学发光峰值和60分积分值均无明显差异(P>0.05)。表明在此预防浓度范围内,亚硒酸钠对吞噬细胞的氧化活性无直接地激发或抑制作用。此为临床补硒及阐明作用机理提供参考依据。更多还原

【Abstract】 Luminol--dependent, zymosan induced whole blood chemiluminescence (whole blood CL) was developed to estimate the oxidizing activity of leucocyte. In this, paper, four different Se concentrations were added to healthy human blood which(0.001μg/ml, 0.01μg/ml, 0.1μg/ml and 1μg/ml)was stored at 4℃ in order to observe chemiluminescence in human leucocyte. The results showed that 1, 12 and 36 hrs after adding Se the peak and integral of whole blood CL did not markedly change (P>0.05). It is included that these four concentrations of Se do not directly stimulate and inhibit the oxidizing activity of leucocyte.更多还原