【摘要】 葡萄糖异构酶细胞经丙酮处理,缓冲液抽提。DEAE—纤维素离子交挟柱层析和Sephadex G—200凝胶柱层析等方法得到分离井纯化。该法与超声破碎法相比,比活提高3.3倍,活力收率为74%,得到了高纯度异构酶,经Sephadex G—200凝胶柱层析获得较高收率的电泳纯制品。更多还原

【Abstract】 Glucose isomerase was purified by means of acetone treatment of cells, buffer extraction, DEAE-Cellulose column chromatography and sephadex-G-200 column chromatography. The processes of acetone treatment and buffer extraction greatly simplified experimental conditions. Special activity was 3.3 times as much as ultrasonic method. The activity yield was 74%. We not only reduced separation steps, but also raised the yield three times. A high yeild electrophoresis pure GI was gained by sephadex-G-200 column chromatography.更多还原