【摘要】 <正> 玫瑰栗色链毒菌(Streptomyces roseocastaneus) No.336变异株对碳源或氮源的利用及其和产葡萄糖异构酶的关系,用半合成培养基进行了摇瓶液体培养试验。测定以15种单糖和糖醇、8种寡糖和5种多糖作为碳源,6种含氮化合物作为氮源对菌的生长和产酶的影响。证明No.336菌株除利用L-阿拉伯糖和D-木糖等五碳糖作为碳源外,也能利用D-葡萄糖等更多还原

【Abstract】 The relationship between utilization of carbon or nitrogen source and enzyme formation in Streptomyces roseocastaneus strain No. 336 with a hemi-synthetic medium has been studied. The strain No. 336 was inoculated into 50 ml of the medium in Erlenmeyer flask of 250 ml capacity and cultivated at 30℃ on a rotary shaker (160-180 r/min).In the 15 monosaccharides and sugar alcohols tested, L-arabinose, D-xylose and D-glucose are found to be most effective carbon source for the formation of the enzyme. Glycerol, xylitol and sorbitol inhibit the yield of enzyme. In the 8 oligosaccharides, cellobiose, maltose and sucrose accelerate the yield of enzyme obviously. In the 5 polysaccharides, inulin, dextrin and starch are used as carbon source. they also accelerate the yield of enzyme in the strain.In the 6 nitrogen sources, corn steep liquor and yeast hydrolysate is superior to polypeptone on the formation of enzyme. The strain can not utilize inorganic nitrogen compounds or ammonium acetate as sole nitrogen source.It is shown that ratio of carbon and nitrogen in medium e.g. 2:1, 1:1 or 3:1 is favourable on the formation of enzyme. If the C/N ratio is decreased, they will promote the growth of the microorganism and decrease the yield of enzyme.The highest activity of D-glucose isomerase (180 u/ml) was obtained in about 4 days by a culture grown with wheat bran hydrolysate -corn steep liquor.更多还原