【摘要】 采用冷冻蚀刻、超薄切片和负染色电镜技术,对Methylomona,sp.761M和Mathylosinus sp.81Z两株甲烷氧化细菌的细微结构进行了观察。在冷冻蚀刻中,细胞从质膜和细胞壁外膜的中央劈开,暴露出四个断裂面。各个断面的结构有所不同,显示出颗粒、小杆、乳状突起、小坑和光滑区等结构。冷冻蚀刻揭示的多层结构,与超薄切片和负染色观察结果一致。此外,对甲烷氧化细菌细胞壁表面图案,细胞内膜结构进行了观察。更多还原

【Abstract】 An electron-microscopic examination of cellular ultrastructure of Methylomonat sp. 761 M and Methylosinus sp. 81 Z was undertaken using the techniques of freeze-etching, ultrathin sectioning and negative staining. When cells were freezeetched, four fracture surfaces, which resulted from splitting down the center of the outer wall membrane and of the plasma membrane, were exposed Various fracture faces with particles, rodlets, holes, nipples and amooth areas were revealed. The muhilayered cell envelope of methanotroph in the freezeetching was similar to observations of thin sections and negative (?)ains. Irregular streak of cell wall, intracytoplasmic membrans were observed too.更多还原